Mouse full-field electroretinograms (ERGs) are dominated by responses of photoreceptors and depolarizing (ON-) bipolar cells, but not much of hyperpolarizing (OFF-) bipolar cells under conventional recording conditions. Here we investigate a novel ERG protocol in mice for functional assessment of the major ON- and OFF-bipolar cell pathways using flicker stimuli for a high luminance with varying frequency up to 30 Hz. Wild-type (WT) and functionally specific transgenic mice (Cnga3^-/-, no cone photoreceptor function; rho^-/-, no rod photoreceptor function; mGluR6^-/-, no ON-bipolar cell function) were examined. The Cnga3^-/- flicker ERG was similar to the WT flicker ERG at very low stimulus frequencies, whereas ERGs were comparable between WT and rho^-/- mice at 5 Hz and above. Between 5 and 15 Hz, ERGs in mGluR6^-/- mice differed in configuration and amplitude from those in WT and rho^-/- mice; in contrast, response amplitudes above 15 Hz were comparable among WT, rho^-/- and mGluR6^-/- mice. In summary, we found three frequency ranges with these conditions that are dominated by activity in the rod pathways (below 5 Hz), cone ON-pathway (between 5 and 15 Hz), and cone OFF-pathway (above 15 Hz) that enables a quick overview of the functionality of the major bipolar cell pathways.