CIPSM - Research Area E

Research Area E - Publications 2020

Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals

24-Nov-2020

Christopher B. Mulholland, Atsuya Nishiyama, Joel Ryan, Ryohei Nakamura, Merve Yiğit, Ivo M. Glück, Carina Trummer, Weihua Qin, Michael D. Bartoschek, Franziska R. Traube, Edris Parsa, Enes Ugur, Miha Modic, Aishwarya Acharya, Paul Stolz, Christoph Ziegenhain, Michael Wierer, Wolfgang Enard, Thomas Carell, Don C. Lamb, Hiroyuki Takeda, Makoto Nakanashi, Sebastian Bultmann, Heinrich Leonhardt

Nat Commun 11, 5972 (2020), https://doi.org/10.1038/s41467-020-19603-1
Nat Commun., online article

carell_lamb_leonhardt_2020_mulholland_natcomm_550.100x0.jpg

Genome-wide DNA demethylation is a unique feature of mammalian development and naïve pluripotent stem cells. Here, we describe a recently evolved pathway in which global hypomethylation is achieved by the coupling of active and passive demethylation. TET activity is required, albeit indirectly, for global demethylation, which mostly occurs at sites devoid of TET ...

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Systematic analysis of the binding behaviourof UHRF1 towards different methyl- and carboxylcytosine modification patterns at CpG dyads

21-Feb-2020

Markus Schneider, Carina Trummer, Andreas Stengl, Peng Zhang, Aleksandra Szwagierczak, M. Cristina Cardoso, Heinrich Leonhardt, Christina Bauer, Iris Antes

PLoS ONE, 15(2): e0229144, https://doi.org/10.1371/journal.pone.0229144
PLoS ONE, online article

The multi-domain protein UHRF1 is essential for DNA methylation maintenance and binds DNA via a base-flipping mechanism with a preference for hemi-methylated CpG sites. We investigated its binding to hemi- and symmetrically modified DNA containing either 5-methylcytosine (mC), 5-hydroxymethylcytosine (hmC), 5-formylcytosine (fC), or 5-carboxylcytosine (caC). Our ...

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