Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins
04-Nov-2013
ChemBioChem, 2013, DOI: 10.1002/cbic.201300435, Volume 14, Issue 16, pages 2114–2118 published on 04.11.2013
Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.
